New Perspectives on Nitrogen Fixation Measurements Using 15N2 Gas
نویسندگان
چکیده
1 Department Biological Oceanography, Leibniz Institute for Baltic Sea Research, Rostock, Germany, 2 Plasma Agriculture, Leibniz Institute for Plasma Science and Technology, Greifswald, Germany, 3 Marine Biological Section, University Copenhagen, Helsingør, Denmark, 4 Aix Marseille Univ, Université de Toulon, CNRS, IRD, MIO UM 110, Marseille, France, 5 Arctic Research Centre, Department of Bioscience, Aarhus University, Aarhus, Denmark, 6 Unisense, Aarhus, Denmark, 7 Georgia Institute of Technology, Atlanta, School of Biological Sciences, Atlanta, GA, United States
منابع مشابه
The Contamination of Commercial 15N2 Gas Stocks with 15N–Labeled Nitrate and Ammonium and Consequences for Nitrogen Fixation Measurements
We report on the contamination of commercial 15-nitrogen (15N) N2 gas stocks with 15N-enriched ammonium, nitrate and/or nitrite, and nitrous oxide. 15N2 gas is used to estimate N2 fixation rates from incubations of environmental samples by monitoring the incorporation of isotopically labeled 15N2 into organic matter. However, the microbial assimilation of bioavailable 15N-labeled N2 gas contami...
متن کاملVariable Nitrogen Fixation in Wild Populus
The microbiome of plants is diverse, and like that of animals, is important for overall health and nutrient acquisition. In legumes and actinorhizal plants, a portion of essential nitrogen (N) is obtained through symbiosis with nodule-inhabiting, N2-fixing microorganisms. However, a variety of non-nodulating plant species can also thrive in natural, low-N settings. Some of these species may rel...
متن کاملThe importance of nodule CO2 fixation for the efficiency of symbiotic nitrogen fixation in pea at vegetative growth and during pod formation
Nodule CO2 fixation is of pivotal importance for N2 fixation. The process provides malate for bacteroids and oxaloacetate for nitrogen assimilation. The hypothesis of the present paper was that grain legume nodules would adapt to higher plant N demand and more restricted carbon availability at pod formation through increased nodule CO2 fixation and a more efficient N2 fixation. Growth, N2 fixat...
متن کاملPrimary products of symbiotic nitrogen fixation. II. Pulse-labelling of serradella nodules with 15N2.
I. By supplying serradella nodules with a pulse of tracer 15N 2, and observing the rate of displacement of excess ~N from certain nitrogen compounds, the role of these compounds in symbiotic nitrogen fixation has been examined. 2. The labelling patterns obtained support the primary position of ammonia as a stable intermediate, and indicate that glutamic acid plus glutamine are the primary amino...
متن کاملSimple approach for the preparation of 15−15N2-enriched water for nitrogen fixation assessments: evaluation, application and recommendations
Recent findings revealed that the commonly used (15)N2 tracer assay for the determination of dinitrogen (N2) fixation can underestimate the activity of aquatic N2-fixing organisms. Therefore, a modification to the method using pre-prepared (15-15)N2-enriched water was proposed. Here, we present a rigorous assessment and outline a simple procedure for the preparation of (15-15)N2-enriched water....
متن کامل